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Anti-cas9 antibody (Vab0001)

Overview

Product name: Anti-Cas9 抗体

Description : 兔多克隆抗体 to Cas9

Tested applications: WB, ICC/IF

Species reactivity Reacts with: Streptococcus pyogenes Cas9

Immunogen:Recombinant full length S.pyogenes Cas9

Positive control WB: E.coli expression of Recombinant Cas9; IF: HEK-293 cells expressing Cas9


Properties

Form: Liquid

Storage instructions: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze /thaw cycle.

Storage buffer:  Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

Purity: Protein A purified

Clonality: polyclonal

Clone number: No.

Isotype: IgG


Applications

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application
Notes
WB1:5000, Detects a band of approximately 155kDa (predicted molecular weight: 162kDa).
ICC/IF1;500
IPNo test
Flow CytNo test
IHC-PNo test
ChIPNo test


Anti-Cas9 antibody Western Blotting

Lane 1 : 1/2000 dilution

Lane 2 : 1/5000 dilution

Secondary:

HRP-conjugated goat anti-rabbit IgG, JIR 115-035-003, 1/5000 dilution

Predicted band size : 160kDa

Observed band size : 150 kDa

Blocking/dilution buffer: 5% NFDM/TBST.


抗体说明书