Anti-cas9 antibody (Vab0001)
Overview
Product name： Anti-Cas9 抗体
Description : 兔多克隆抗体 to Cas9
Tested applications： WB, ICC/IF
Species reactivity Reacts with: Streptococcus pyogenes Cas9
Immunogen：Recombinant full length S.pyogenes Cas9
Positive control WB: E.coli expression of Recombinant Cas9; IF: HEK-293 cells expressing Cas9
Properties
Form: Liquid
Storage instructions: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze /thaw cycle.
Storage buffer: Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity: Protein A purified
Clonality: polyclonal
Clone number: No.
Isotype: IgG
Applications
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
| WB | 1:5000, Detects a band of approximately 155kDa (predicted molecular weight: 162kDa). |
| ICC/IF | 1;500 |
| IP | No test |
| Flow Cyt | No test |
| IHC-P | No test |
| ChIP | No test |
Anti-Cas9 antibody Western Blotting Lane 1 : 1/2000 dilution Lane 2 : 1/5000 dilution Secondary: HRP-conjugated goat anti-rabbit IgG, JIR 115-035-003, 1/5000 dilution Predicted band size : 160kDa Observed band size : 150 kDa Blocking/dilution buffer: 5% NFDM/TBST. |
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